Monomer (By similarity). Interacts with YWHAZ; the interaction requires phosphorylation on Thr-29, and modulates the enzymatic activity of AANAT through preventing dephosphorylation and/or proteolysis and stabilizing substrate binding. Subsequently, a second molecule of AANAT can bind, via the phosphorylated Ser-203 site, the other YWHAZ monomer with similar effect.
Function:
Catalyzes the N-acetylation of serotonin into N-acetylserotonin.
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s
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤6.8%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤10.7%
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤3.9%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.7%
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Recovery
Recovery was determined by spiking various levels of SNAT into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
99
94-100
Plasma
98
96-102
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Recovery was determined by spiking various levels of ACE into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
99
91-103
Plasma
98
93-106
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Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SNAT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1：2
1:4
1:8
1:16
serum(n=5)
92-105%
90-108%
95-102%
92-107%
EDTA plasma(n=5)
94-103%
96-101%
93-105%
95-102%
heparin plasma(n=5)
93-104%
94-104%
95-103%
91-105%
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1：2
1:4
1:8
1:16
serum(n=5)
92-105%
90-101%
95-105%
92-109%
EDTA plasma(n=5)
89-95%
93105%
89-94%
90-106%
heparin plasma(n=5)
93-104%
94-110%
101-111%
91-110%
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Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA];TISSUE SPECIFICITY;INDUCTION;IDENTIFICATION OF A TRUNCATED FORM IN C57BL/6
Vaild Sequence:
